Journal: bioRxiv

Article Title: Radiotherapy Enhancement by Gold Nanocluster-functionalized Nanoliposomes Using Polychromatic Orthovoltage X-ray Irradiation

doi: 10.64898/2025.12.04.692299

Figure Lengend Snippet: (A) Brightfield images and corresponding viability heatmaps of microtumors incubated with AuLPs with 0 to 10 mol% of AuDDT without irradiation (0 Gy), and at 8 Gy (B), scale bar 400µm. (C) Quantification of microtumor viability in relation to AuDDT loaded on AuLPs for PANC-1 microtumors at different Xrays doses, 0, 4 and 8 Gy (Data was fitted with simple linear regression). (D) Quantification of normalized microtumor size in relation to AuDDT loaded on AuLPs for PANC-1 microtumors at different Xrays doses, 0, 4 and 8 Gy (curve was fitted for inhibitor vs normalized response-variable slope – four parameters). (E) Residual viable disease as the live fraction area remaining per condition, as AuDDT loaded on AuLPs (2mM) dose response for the different Xrays doses 0, 4 and 8 Gy. Data represent a sample sizes of n = 109-348.

Article Snippet: Human pancreatic cancer cell lines PANC-1 (CRL-1469) and MIA PaCa-2 (CRL-1420) were obtained from the American Type Culture Collection (ATCC) and used between passages 2 and 30.

Techniques: Incubation, Irradiation

Journal: bioRxiv

Article Title: Radiotherapy Enhancement by Gold Nanocluster-functionalized Nanoliposomes Using Polychromatic Orthovoltage X-ray Irradiation

doi: 10.64898/2025.12.04.692299

Figure Lengend Snippet: (A) ICP-MS quantification of Au content in spleen, liver and tumor, at 3h, 5h and 24h post liposome injection. (B) BPD fluorescence signal in spleen, liver and tumor, at 3h, 5h and 24h post liposome injection. AuDDT signal intensity in spleen, liver and tumor, at 3h, 5h and 24h post liposome injection. Data represents n=9 from three technical repetitions. (D) Elemental mapping of orthotopic PANC-1 tumor sections by LIBS for quantification of phosphorus (P) and gold (Au) on Au+BPD treated mice, depicted are tumor tissue, at 3h, 5h and 24h post liposome injection and representative H&E staining of the tissue slide. Scale bar, 2 mm.

Article Snippet: Human pancreatic cancer cell lines PANC-1 (CRL-1469) and MIA PaCa-2 (CRL-1420) were obtained from the American Type Culture Collection (ATCC) and used between passages 2 and 30.

Techniques: Injection, Fluorescence, Staining

Journal: Journal of Translational Medicine

Article Title: Tumor marker–guided precision BNCT for CA19-9–positive cancers: a new paradigm in molecularly targeted chemoradiation therapy

doi: 10.1186/s12967-025-07349-7

Figure Lengend Snippet: Biosynthesis pathway of carbohydrate antigen 19–9 (CA19-9) and clinical relevance of fucosyltransferase expression. ( A ) schematic illustration of the CA19-9 biosynthetic pathway. ( B ) chemical structures of CA19-9 and its monosaccharide component, L-fucose. ( C ) comparative transcriptomic analysis using the cancer cell Line encyclopedia (ccle): gene expression profiles in CA19-9–high versus CA19-9–normal cell lines (q < 0.01). ( D ) rna sequencing of pancreatic tumor samples comparing CA19-9–high (poor prognosis) and CA19-9–low (favorable prognosis) cases. ( E ) Kaplan–Meier survival analysis of pancreatic ductal adenocarcinoma (pdac) patients stratified by FUT3 expression. ( F ) Kaplan–Meier survival analysis of lung adenocarcinoma patients stratified by FUT3 expression

Article Snippet: Human pancreatic cancer cell lines (PANC-1, AsPC-1, Panc 04.03, and PK-45 H), cholangiocarcinoma (HuCCT-1), Krukenberg tumor (HSKTC), and ovarian cancer (OVISE) cell lines were obtained from ATCC, RIKEN BRC, or JCRB Cell Bank and cultured according to their protocols.

Techniques: Expressing, Gene Expression, RNA Sequencing